Characterization of an Immobilized Amino Acid Racemase for Potential Application in Enantioselective Chromatographic Resolution Processes

Enantioselective resolution processes can be improved by integration of racemization. Applying environmentally friendly enzymatic racemization under mild conditions is in particular attractive. Owing to the variety enzymes and progress enzyme engineering, suitable racemases found for many chiral systems. An amino acid racemase (AAR) from P. putida KT2440 capable processing a broad spectrum acids at fast conversion rates. The focus this study evaluation potential integrating AAR immobilized on Purolite ECR 8309 racemize L- or D-methionine (Met) within an enantioselective chromatographic process. Racemization rates were studied different temperatures, pH values, fractions organic co-solvents. long-term stability operating storage was excellent recyclability using water with up 5 vol% ethanol 20 °C could demonstrated. Packed as fixed bed reactor, coupled processes; instance, chromatography preferential crystallization. performance coupling it estimated quantitatively, exploiting parametrized sub-models. To indicate large AAR, are finally given lysine, arginine, serine, glutamine, asparagine.